Recombinant Human BMP-2

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRB100C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Bone Morphogenetic Protein 2 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are currently more than thirteen BMPs that have been discovered, and they are involved in inducing cartlage and bone formation. BMP-2 is expessed in a variety of tissues including lung, spleen, brain, liver, prostate, ovary and small intestine. It is a potent osteoninductive cytokine and functions in association with osteoconductive carriers such as collagen and synthetic hydroxapatite. The functional form of BMP-2 is a disulfide-linked homodimer of approximately 26 kDa. The precursor of BMP-2 is a 259 aa sequence containing the 23 aa N-terminal signal sequence which will be cleaved by a furin-type protease to form the mature peptide. Recombinant Human BMP-2 is a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptde chains.
Formulation
Lyophilized from a 0.2 um sterile filtered solution containing 10 mM Sodium Citrate, pH 3.5
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
GeneID
650
Source
E. coli
Molecular Weight
ca. 26 kDa
Purity
>95% by SDS-PAGE and HPLC
Specific Activity
>5.0 x 10E3 IU/mg.
Endotoxin Level
<1 EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to the standard. The ED50 determined by inducing alkaline phosphatase production of mouse ATDC5 cells is < 200 ng/ml.
AA Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Arbeitsanleitung

CS-CRB100C.pdf

Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 2.988,60 €
Preis: 2.988,60 €
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