|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
25 ug |
|
ArtNr |
CS-CRC012B |
|
Eclass 6.1 |
34160400 |
|
Eclass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
Recombinant human CTLA-4/CD152 is a homodimeric, glycosylated polypeptide chain. Each subunit (40 kDa) is fused to a polypeptide linker to the Fc portion of human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses, in vivo . Large doses of this soluble protein also suppress responses to a second immunization. |
| Formulation |
Lyophilized from a sterile filtered solution without additives |
| Reconstitution |
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions. |
| Storage and Stability |
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week in working aliquots. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles. |
| GeneID |
1493 |
| Source |
Sf9 cells |
| Molecular Weight |
80.0 kDa |
| Purity |
> 95% as determined by RP-HPLC and SDS-PAGE analyses |
| Endotoxin Level |
< 0.1 ng/ug of CTLA4 |
| AA Sequence |
The sequence of the first five N-terminal amino acids is Lys-Ala-Met-His-Val. |
