Recombinant Human CYR61

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 20 ug
Host E.coli
ArtNr CS-CRC023B
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Cysteine-rich angiogenic inducer 61 (Cyr61) encoded by the Cyr61 gene is a dynamically expressed, multifunctional matricellular protein and it is also a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family. Cyr61 plays essential roles in cardiovascular development during embryogenesis and regulates inflammation, wound healing and fibrogenesis in the adult. Aberrant CCN1 expression is associated with myriad pathologies, including various cancers and diseases associated with chronic inflammation. Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. Cyr61 consists of four domains. There are an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
3491
Source
E. coli
Molecular Weight
39.5 kDa
Purity
> 95% as determined by SDS-PAGE and HPLC analyses
Specific Activity
> 3.3 x 10E5 IU/mg.
Endotoxin Level
Less than 1 EU/ug of rHuCYR61 as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse Balb/3T3 cells is less than 3.0 ug/ml/
AA Sequence
TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 318,01 €
Rabatt: -10,0%
Preis: 286,21 €
Sie sparen: 31,80 €
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Arbeitsanleitung

CS-CRC023B.pdf