Recombinant Human CCAAT/enhancer binding protein gamma (aa 39-147) His

CCAAT/enhancer binding protein(C/EBP) g is a family of transcription factors all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP a, b, g, d, that form homodimers and/or form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein, a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBP g may cooperate with Fos to bind PRE- enhancer elements. CEBP-g Recombinant Human His-Tag fusion protein produced in, E. coli, is a single, non-glycosylated polypeptide chain containing amino acids 146 (aa 39-147) and having a MW = 16.5 kDa. The DNA binding domain of CEBP-g was purified by proprietary chromatographic techniques.

Sterile filtered liquid in 20 mM Tris-HCl, pH 7.5 + 0.1 M NaCl + 5 mM beta-mercaptoethanol

Store at 2-4C for up to 4 weeks or in working aliquots at -20C for longer storage. Add a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.