Recombinant Human Desmin

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 20 ug
Host E.coli
ArtNr CS-CRD114B
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Formulation
Desmin was lyophilized from a 1 mg/ml solution containing 30 mM Tris-HCl, pH 8 + 9.5 M urea + 2 mM DTT + 2 mM EDTA and 10 mM methylammonium chloride.
Reconstitution
Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized Desmin in sterile 18 MU-cm water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage and Stability
Lyophilized protein is stable at room temperature for 3 weeks, but it is recommended to store the lyophilized product desiccated at -20C to -80C. Upon reconstitution, protein should be stored at 2-4C for one week and for future use at -20C to -80C. Add a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
1674
Source
E. coli
Molecular Weight
53, 539 dalton.
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Level
<0.1 ng/ug of recombinant Desmin

Arbeitsanleitung

CS-CRD114B.pdf

Menge: 20 ug
Lieferbar: In stock
Listenpreis: 428,68 €
Preis: 428,68 €
lieferbar

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Arne Pelz

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