Recombinant Human FGF Basic

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host E.coli
ArtNr CS-CRF001B
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Human Fibroblast Growth Factor basic (FGF2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF, and KGF (keratinocyte growth factor, FGF-7). All factors are products of different genes, some of which are oncogene products (FGF-3, FGF-4, FGF-5).
Formulation
Lyophilized from a sterile filtered solution in PBS, pH 7.4
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, divide the reconstituted product into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
2247
Source
E. coli
Molecular Weight
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
Purity
>96.0% by HPLC and SDS-PAGE
Specific Activity
1.7 x 106 units/mg
Endotoxin Level
<0.1 ng/ug FGF2
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using mouse NR6R/3T3 cells is less than 0.6 ng/ml.
AA Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Arbeitsanleitung

CS-CRF001B.pdf

Menge: 50 ug
Lieferbar: In stock
Listenpreis: 295,22 €
Preis: 295,22 €
lieferbar

Angebot anfordern

Fragen zum Produkt?

Ihr Ansprechpartner:

Arne Pelz

sales@hoelzel.de