Recombinant Human FGF2/FGF-basic

This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, divide the reconstituted product into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.

Human Fibroblast Growth Factor basic (FGF2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF, and KGF (keratinocyte growth factor, FGF-7). All factors are products of different genes, some of which are oncogene products (FGF-3, FGF-4, FGF-5).

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using mouse NR6R/3T3 cells is less than 0.6 ng/ml.