Recombinant Human FGF-21

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRF159C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28 a.a. signal sequence and a 181 a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81% a.a. identity to mouse and rat FGF-21, and is known to be active on mouse cells. Recombinant Human FGF-21 is a single non-glycosylated polypeptide chain containing 181 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
GeneID
26291
Source
E. coli
Molecular Weight
ca.19.5 kDa
Purity
>95% by SDS-PAGE and HPLC analyses.
Specific Activity
>1.7 x 10E3 IU/mg in the presence of 5 ug of recombinant mouse Klotho-beta
Endotoxin Level
<0.1 ng/ug of protein.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse NIH/3T3 cells is less than 600 ng/ml.
AA Sequence
HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 1.955,88 €
Rabatt: -10,0%
Preis: 1.760,29 €
Sie sparen: 195,59 €
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Arbeitsanleitung

CS-CRF159C.pdf