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Recombinant Human GCSF

ArtNr CS-CRG300A
Hersteller Cell Sciences
Menge 2 ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host E.coli
Purity >98% by SDS-PAGE and HPLC
Sequence TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products C17orf33, GCSF, MGC45931, filgrastim, lenograstim, pluripoietin, CSF3OS, granulocyte colony-stimulating factor
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Storage Conditions
Stable at 2-8C, but best kept desiccated -20C. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
Molecular Weight
18.7 kDa
Description
Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-g. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-a activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity. Recombinant Human Granulocyte-Colony Stimulating Factor is a single non-glycosylated polypeptide chain containing 174 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 10 mM sodium acetate buffer + 5% trehalose, pH 4.0.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Specificity
>1 x 107 IU/mg
Biological Activity
Recombinant Human G-CSF is fully biologically active when compared to standard. The ED50, determined by a cell proliferation assay using m, pise NFS-60 cells is less than 0.1 ng/ml.
Endotoxin Level
Less than 1EU/ug as determined by LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 ug
Lieferbar: In stock
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