Recombinant Human IGFBP5

Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGFBP 5 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 ug/ml in the presence of 15 ng/ml of Human IGF-II corresponding to a Specific Activity of 66, 667IU/mg.