Recombinant Human sVEGFR-2D1-7/Fc Chimera

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
Host Insect
ArtNr CS-CRK100A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant human soluble Vascular Endothelial Growth Factor Receptor-2 (sVEGFR-2D1-7) was fused with the Fc part of human IgG1. The recombinant mature sVEGFR-2D1-7/Fc is a disulfide-linked homodimeric protein. The sVEGFR-2D1-7/Fc monomers have a mass of approximately 160 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Ala757), which contain all the information necessary for high affinity ligand binding. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
Formulation
Lyophilized from Tris, pH 7.2.
Reconstitution
Centrifuge vial prior to opening. The lyophilized sVEGFR-2/Fc is soluble in water and most aqueous buffers. The lyophilized sVEGFR-2/Fc should be reconstituted in PBS or medium to a concentration not lower than 50 ug/ml.
Storage and Stability
Lyophilized samples are stable for greater than six months at -20C to -80C. Reconstituted sVEGFR-2/Fc should be stored in working aliquots at -20C to -80C. Avoid repeated freeze-thaw cycles.
GeneID
3791
Source
Insect cells
Molecular Weight
145 kDa (Monomer)
Purity
>90% as determined by SDS-PAGE and visualized by silver stain
Protein Accession No
NP_002244
Endotoxin Level
<0.1 ng/ug of sVEGFR-2
Biological Activity
Determined by its ability to inhibit the VEGF-dependent proliferation of human uMbilical vein endothelial cells. The ED50 is typically 10.0-30.0 ng/ml.
AA Sequence
ASVGLPSVSL DLPRLSIQKD ILTIKANTTL QITCRGQRDL DWLWPNNQSG SEQRVEVTEC SDGLFCKTLT IPKVIGNDTG AYKCFYRETD LASVIYVYVQ DYRSPFIASV SDQHGVVYIT ENKNKTVVIP CLGSISNLNV SLCARYPEKR FVPDGNRISW DSKKGFTIPS YMISYAGMVF CEAKINDESY QSIMYIVVVV GYRIYDVVLS PSHGIELSVG EKLVLNCTAR TELNVGIDFN WEYPSSKHQH KKLVNRDLKT QSGSEMKKFL STLTIDGVTR SDQGLYTCAA SSGLMTKKNS TFVRVHEKPF VAFGSGMESL VEATVGERVR IPAKYLGYPP PEIKWYKNGI PLESNHTIKA GHVLTIMEVS ERDTGNYTVI LTNPISKEKQ SHVVSLVVYV PPQIGEKSLI SPVDSYQYGT TQTLTCTVYA IPPPHHIHWY WQLEEECANE PSQAVSVTNP YPCEEWRSVE DFQGGNKIEV NKNQFALIEG KNKTVSTLVI QAANVSALYK

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Menge: 10 ug
Lieferbar: In stock
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