Recombinant Human KU P70/P80 His

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.

Ku antigen consists of two polypeptides (70 kDa and 80 kDa, the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20% of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10% of SLE and MCTD patients, and in up to 20% of patients with primary Sjogren's syndrome. In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a MW = 70, 638 Dalton for the p70 subunit and 83, 528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

0.2-0.6 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.