Recombinant Human Leptin

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 mg
Host E.coli
ArtNr CS-CRL300C
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. It is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus), (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of H-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3-36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.
Formulation
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
3952
Source
E. coli
Molecular Weight
Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Purity
>97% by SDS-PAGE and HPLC analyses.
Specific Activity
>5.0 x 10E5 IU/mg
Endotoxin Level
Less than 1EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human Leptin R transfected BaF3 mouse proB cells is less than 2 ng/ml.
AA Sequence
VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

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Menge: 5 mg
Lieferbar: In stock
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