Recombinant Human Omentin/Intelectin-1

Store lyophilized protein at -20C. After reconstitution, protein is stable at 2-4C for 1 week. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA), aliquot, and freeze at -20C. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Recombinant Human Omentin/Intelectin-1 is a single, 313 aa polypeptide protein purified by proprietary chromatographic techniques. Omentin/Intelectin-1 is a recently recognized gene highly localized to omental tissue (visceral adipose tissue). Omentin is present in the stromal vascular cells in the adipose tissue rather than in the adipocytes. Omentin is predominantly expressed in the visceral adipose tissue than the subcutaneous tissue, with the omentin mRNA being 150 times higher in the visceral adipose tissue. Omentin has also been detected in human blood using western blot analysis, and seems to increase insulin-stimulated glucose uptake in 3T3-L1 adipocytes in mice. Omentin seems to increase Akt phosphorylation irrespective of insulin presence. Its role in glucose metabolism and obesity remains to be described, an insulin-sensitizing action is possible. Differences in Omentin expression has been noted in the adipose tissue of healthy humans and patients with inflammatory bowel disease although its significance is unknown.

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized Omentin in sterile distilled water to a concentration not less than 100 ug/ml, which can then be further diluted in other aqueous solutions.