|
Hersteller |
Cell Sciences
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
10 ug |
|
Host |
E.coli |
|
ArtNr |
CS-CRS000B |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
SDF-1alpha and SDF-1beta, members of the chemokine alpha subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1alpha and SDF-1beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1beta and absent from SDF-1alpha. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils. Recombinant Human SDF-1 alpha is a single, non-glycosylated polypeptide chain consisting of 68 amino acids. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.0 + 130 mM NaCl. |
| Reconstitution |
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions. |
| Storage and Stability |
This lyophilized preparation is stable at 2-8C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles. |
| GeneID |
6387 |
| Source |
E. coli |
| Molecular Weight |
8.0 kDa |
| Purity |
>97.0% by RP-HPLC and SDS-PAGE |
| Specific Activity |
>1.1 x 105 IU/mg |
| Endotoxin Level |
Less than 1EU/ug as determined by LAL method |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is less than 9 ng/ml. |
| AA Sequence |
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
