Recombinant Human VEGF 121

Lyophilized Vascular Endothelial Growth Factor-121 should be stored desiccated below -20C. Upon reconstitution VEGF-121, may be stored at 2-4C for 1 week. For future use, aliquot and store below -20C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. in vitro , VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in the VEGFA gene have been associated with proliferative and nonproliferative diabetic retinopathy. Recombinant Human Vascular Endothelial Growth Factor-121 is a double, non-glycosylated, polypeptide chain containing 121 amino acids. VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. Recombinant VEGF-121 is purified by proprietary chromatographic techniques.

Reconstitute the protein in sterile distilled water at not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.