Recombinant Human Vimentin

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
Host E.coli
ArtNr CS-CRV020B
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGF beta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.
Formulation
Lyophilized from a sterile filtered solution containing 30 mM Tris-HCl, pH 8 + 9.5 M urea + 2 mM EDTA + 2 mM DTT + 10 mM methylammonium chloride
Reconstitution
After vimentin is dissolved in 9.5 M urea buffer, protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Storage and Stability
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week, at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
Concentration
1mg/ml
GeneID
7431
Source
E. coli
Molecular Weight
53.7 kDa (calculated from sequence), 57.0 kDa (determined by SDS gel electrophoresis)
Purity
> 95% as determined by SDS gel electrophoresis
Endotoxin Level
< 0.1 ng/ug of VIM
AA Sequence
mstrsvssss yrrmfggpgt asrpsssrsy vttstrtysl gsalrpstsr slyasspggv yatrssavrl rssvpgvrll qdsvdfslad aintefkntr tnekvelqel ndrfanyidk vrfleqqnki llaeleqlkg qgksrlgdly eeemrelrrq vdqltndkar veverdnlae dimrlreklq eemlqreeae ntlqsfrqdv dnaslarldl erkveslqee iaflkklhee eiqelqaqiq eqhvqidvdv skpdltaalr dvrqqyesva aknlqeaeew ykskfadlse aanrnndalr qakqesteyr rqvqsltcev dalkgtnesl erqmremeen faveaanyqd tigrlqdeiq nmkeemarhl reyqdllnvk maldieiaty rkllegeesr islplpnfss lnlretnlds lplvdthskr tlliktvetr dgqvinetsq hhddle

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Menge: 10 ug
Lieferbar: In stock
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