Recombinant Human Alanyl-tRNA Synthetase His

Store at 2-4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.

Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes that interpret the RNA code and attach specific amino acids to the tRNAs that contain the cognate trinucleotide anticodons. The AARS protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop. Recombinant Human Alanyl-tRNA Synthetase produced in Sf9 cells is a glycosylated, polypeptide chain having a MW = 107.6 kDa. It is expressed with a -6x His tag and purified by proprietary chromatographic techniques.