Recombinant Human Ubiquitin-Conjugating Enzyme E2C His

Lyophilized UBE2C should be stored desiccated below -20C. Upon reconstitution, UBE2C may be stored at 2-4C for 1 week and for future use, store below -20C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

UBE2C is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis. This activity is essential at the end of mitosis for the inactivation of the partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBE2C bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBE2C is useful for, in vitro, ubiquitinylation reactions. Recombinant Human Ubiquitin-Conjugating enzyme E2C is a 21.1 kDa protein containing 191 amino acids, including a 6x His tag. It is purified by proprietary chromatographic techniques.

Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.