Recombinant Human CDC42 (G12V mutant aa 1-178) GST *di

Stable for 1 year in working aliquots at -80aC. Avoid repeated freeze-thaw cycles.

Rho family GTPases Rac1 and Cdc42 (cell division cycle 42) belong to the Ras superfamily of small GTP-binding proteins. The human homolog of yeast Cdc42 is essential for cell polarity and regulates cytoskeletal rearrangements in responses to growth factor stimulation. The C-terminal deletion of 13 amino acids of Cdc42 ÄC includes the polybasic domain consisting of six contiguous basic amino acids. The polybasic domain of Cdc42 is required for homodimer formation. The Gly12 to Val mutation of Cdc42 ÄC leads to a constitutive active protein. The GST-Tag facilitates the proteins application in typical GST pull-down assays.