Recombinant Human Small Ubiquitin Related M fier 2

Stable at 2-4C for one week, but should be stored dessicated at -20C to -80C if not reconstituted upon arrival. Reconstituted protein should be aliquoted and stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. Unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All SUMO proteins share the conserved ubiquitin domain and the C-terminal diglycine cleavage/attachment site. Human SUMO2, also known as Sentrin2 and SMT3B is synthesized as a 95 amino acid (aa), 11 kDa propeptide that contains a two aa C-terminal prosegment, and an 18 aa N-terminal protein interacting region (aa 33 -50). Following prosegment cleavage, the C-terminal glycine is enzymatically attached to a lysine on a target protein. Human SUMO2 shares 100% sequence identity to SUMO-2 from mouse. SUMO2 also has very high sequence homology to SUMO3 and SUMO4, 86% and 85%, respectively. SUMO2 shares only 44% sequence identity to SUMO1. The SUMO-2 is purified by proprietary chromatographic techniques.