Native Mouse Fibrinogen *di

Fibrinogen, also called Factor I, is the principal protein of vertebrate blood clotting forming a hexamer containing two sets of three different chains (alpha, beta, and gamma), linked to each other by disulfide bonds. The N-terminal sections of these three chains contain the Cysteines that participate in the cross-linking of the chains. The C-terminal parts of the alpha, beta and gamma chains contain a domain of about 225 amino acid residues, which can function as a molecular recognition unit, engage in protein-protein interactions, and bind carbohydrates. On the Fibrinogen alpha and beta chains, there is a small peptide sequence (called a Fibrinopeptide) that prevents Fibrinogen from spontaneously forming polymers with itself. Fibrinogen is the precursor of Fibrin. Fibrin, also called Factor Ia, is a fibrous protein involved in the clotting of blood, and is non-globular. Native Mouse Fibrinogen is prepared from fresh mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography.

Frozen liquid in 0.02 M Sodium Citrate-HCl, pH 7.4

Store product at -80C. Stable for 3 years from delivery. Thaw protein in a 37C water bath without agitation until completely liquid. Then, gently mix before use. Keep fibrinogen at 25-37C when in use. Aliquot and freeze the unused portion at -80C. Avoid repeated freeze-thaw cycles.