Vergleich

Recombinant Mouse Sonic Hedgehog

ArtNr CS-CRS161B
Hersteller Cell Sciences
Menge 25 ug
Kategorie
Typ Proteins
Specific against Mouse (Murine, Mus musculus)
Host E.coli
Purity Typically 97% determined by: Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm
Sequence MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products Shh, SHH, HHG-1, HHG1, Dsh, Sonic hedgehog protein., Hx, Hhg1, Hxl3, M100081, 9530036O11Rik
Lieferbar
Manufacturer - Category
Biomolecules
Storage Conditions
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Avoid repeated freeze-thaw cycles. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed.
Molecular Weight
19.8 kDa
Description
Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors. Recombinant mouse SHH is a non-glycosylated protein, containing 176 amino acids. The Cys at position 25 has been substituted with Ile.
Formulation
Recombinant mouse SHH is lyophilized from 10 mM Na2PO4, pH 7.5.
Reconstitution
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Biological Activity
The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.6-3 ug/mL
Endotoxin Level
Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
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