Recombinant Mouse Thrombopoietin

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Mouse
Menge 1.0 mg
Host E.coli
ArtNr CS-CRT401C
eClass 6.1 34160400
eClass 9.0 42020190
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Description
Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain).
Formulation
Recombinant mouse TPO is lyophilized with no additives.
Reconstitution
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Storage and Stability
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
21832
Source
E. coli
Molecular Weight
18.7 kDa
Purity
95% as determined by reducing and non-reducing SDS-PAGE
Protein Accession No
P40226
Endotoxin Level
<=1EU/ug protein as measured by kinetic LAL analysis.
Biological Activity
The activity is determined by the dose-dependent stimulation of MO7e cells and is typically less than 1 ng/mL.
AA Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

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Menge: 1.0 mg
Lieferbar: In stock
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