Recombinant Viral SRC/SRC kinase GST Active *di

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 5 ug
Host Insect
ArtNr CS-CRS124A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant full length Rous sarcoma virus Src protein containing N-terminal GST tag was expressed by baculovirus in Sf9 insect cells. Src family belongs to non-receptor tyrosine kinases. Src was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. Src is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression. Oncogenic forms of the Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that Src plays a role in these functions by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquitin ligase that controls Src protein levels. These studies also shed light on the role of focal adhesion kinase (FAK) downstream of v-Src and other signaling pathways in controlling migration, invasion and survival of transformed cells. Src directly phosphorylates integrins and can also modulate R-Ras activity. Moreover, it stimulates the E-cadherin regulator Hakai, interacts with and phosphorylates the novel podosome-linked adaptor protein Fish, and progressively phosphorylates the gap junction component connexion 43. In addition to the above functions and substrates, the role in cellular physiology of Src is continually expanding.
Formulation
Recombinant protein in storage buffer (50 mM Tris-HCl + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mM EDTA + 0.1 mM PMSF + 25% glycerol, pH, 7.5).
Storage and Stability
Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
Concentration
0.1 mg/ml
Source
Sf9 insect cells
Purity
2 ug of Src protein was subjected to SDS-PAGE and Coomassie blue staining. The scan of the gel showed >90% purity of the Src 1 product, and the band was at ca.85 kDa (Fig. 2).
Specific Activity
116 nmol/min/mg: 116 nmol phosphate incorporated into Src substrate peptide per minute per mg proteins at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay in 25 ul reaction volume). See QA/QC section for details.

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Menge: 5 ug
Lieferbar: In stock
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