Recombinant Orf Virus VEGFE His

Kein Verkauf an Patienten, Ärzte, Apotheken oder Privatpersonen! Mehr erfahren

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 5 ug
Host E.coli
ArtNr CS-CRV007B
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (GenBank accession No. AF106020) was expressed in, E. coli, as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized, in vitro, and has a predicted MW ca.35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, however in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt -1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor-2/KDR.
Formulation
Lyophilized from a buffer of PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. The lyophilized VEGF-E is soluble in water and most aqueous buffers. The lyophilized VEGF-E should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50.0 ug/ml.
Storage and Stability
Lyophilized samples are stable for greater than six months at -20C to -80C. Reconstituted VEGF-E should be stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles.
Source
E. coli
Purity
> 90%, by SDS-PAGE and visualized by silver stain
Specific Activity
2 x 105 units/mg
Endotoxin Level
< 0.1 ng per ug of VEGF-E
Biological Activity
Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1-5 nglmL.
AA Sequence
MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 5 ug
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen