Recombinant Yeast Thioredoxin

TRX although stable at 4C for 3 weeks, should be stored desiccated below -18C. Please prevent freeze thaw cycles.

Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Thioredoxin Yeast Recombinant produced in, E. coli, is a single, non-glycosylated, polypeptide chain.

It is recommended to reconstitute the lyophilized TRX in sterile 18 MU-cm water.