Vergleich

Native Porcine ECGF

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Pig
Menge 6 mg
Host Porcine
ArtNr CS-CSI11673
Targets TYMP, FGF1
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Biological Activity
Optimum concentration for human uMbilical vein endothelial cells (HUVEC) range from 100-300 ug/ml, optimal concentration with heparin (50 ug/ml) is at 20 ug/ml. As a growth supplement for use in mAb production the optimum range is 25-100 ug/ml.
Description
Endothelial cell growth factor (ECGF) is an extract of pig brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. Endothelial cells from human uMbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells, in vitro, can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.
Formulation
Lyophilized from PBS, pH 7.4 without preservatives.
Reconstitution
Centrifuge vial prior to opening. Endothelial cell growth factor is supplied as a sterile lyophilized powder containing 15 mg protein per vial. To obtain a stock solution reconstitute the contents of the vial in 5 ml of pre warmed (37C) sterile balanced salt solution. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. Although the stock solution can be added aseptically to sterile tissue culture medium, it is recommended that medium containing diluted product be aseptically filtered prior to use.
Storage and Stability
Store lyophilized product at 2-4C. After reconstitution, the product may be stored as aliquots at -20C. Avoid repeated freeze-thaw cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 6 mg
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 18.04.2024 

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