Recombinant Rat VEGF-C aa C152S Mutation His

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Rat
Menge 2 ug
Host Insect
ArtNr CS-CRV009A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
VEGF-C aa C152S is a point mutant generated by the replacement of the second conserved Cys residue at aa 152 of the recombinant processed VEGF-C by a Ser residue. VEGF-C C152S is an analog to the human VEGF-C 156 mutant and is only active toward VEGFR-3/FLT-4. However, unlike wild type VEGF-C, the aa 152 mutant is unable to bind to and to activate signaling through VEGFR-2/KDR. VEGF-C C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that the VEGF-like effects of VEGF-C require VEGFR-2 binding. Recombinant Rat VEGF-C 152 contains 152 amino acid residues fused to a 6x His tag at the C-terminal end. As a result of glycosylation, VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Formulation
The protein was lyophilized from a concentrated (1 mg/ml) solution with BSA.
Reconstitution
It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile distilled water at not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage and Stability
Lyophilized Vascular Endothelial Growth Factor-C152S should be stored desiccated below -20C. Upon reconstitution, VEGF-C 152 may be stored at 2-4C between 2-7 days. For future use, store below -20C. Avoid repeated freeze-thaw cycles.
GeneID
114111
Source
Sf9 Insect Cells.
Purity
> 90.0% as determined by RP-HPLC and SDS-PAGE.
Biological Activity
Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in pig aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6 KUnits/mg.

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Menge: 2 ug
Lieferbar: In stock
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