Recombinant Rat VEGF-C aa C152S Mutation His

VEGF-C aa C152S is a point mutant generated by the replacement of the second conserved Cys residue at aa 152 of the recombinant processed VEGF-C by a Ser residue. VEGF-C C152S is an analog to the human VEGF-C 156 mutant and is only active toward VEGFR-3/FLT-4. However, unlike wild type VEGF-C, the aa 152 mutant is unable to bind to and to activate signaling through VEGFR-2/KDR. VEGF-C C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that the VEGF-like effects of VEGF-C require VEGFR-2 binding. Recombinant Rat VEGF-C 152 contains 152 amino acid residues fused to a 6x His tag at the C-terminal end. As a result of glycosylation, VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile distilled water at not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.