BT-20 Cells

The BT-20 cell line is a human breast adenocarcinoma cell line that was established in 1958 from the malignant tissue of a 74-year-old Caucasian female patient. This cell line exhibits epithelial-like morphology and is often used in research focused on breast cancer biology, particularly in studies exploring the hormonal regulation of cancer growth, gene expression, and the efficacy of therapeutic agents against breast cancer.

BT-20 cells are characterized by their ability to form tumors when implanted in immunocompromised mice, thus serving as a useful in vivo model for breast cancer. These cells express receptors for estrogen, progesterone, and androgen, making them relevant for studies on hormone response pathways. Additionally, genetic analysis of BT-20 cells has revealed mutations in genes such as TP53 and PIK3CA, which are common in breast cancer, supporting their use in genetic and pharmacological research.

In vitro, BT-20 cells are used to study the mechanisms of cancer cell proliferation, migration, and invasion. They are also employed to assess the cytotoxicity of chemotherapy agents, making them critical for preclinical testing of anti-cancer drugs. The adaptability of BT-20 cells to various culture conditions and their robust growth in vitro make them a valuable resource for cancer research laboratories focusing on the underlying mechanisms of breast cancer and the development of new therapeutic strategies.

Monolayer, adherent

74 years

Caucasian

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in nude mice. Forms grade II adenocarcinomas

1 x 10^4 cells/cm^2 will yield in a confluent layer in about 6 days

negative

modal number = 50, many markers with large subtelocentrics most characteristic. (P87) Hyperdiploid with abnormalities including fragmented chromosomes, breaks, secondary constrictions, translocations, submetacentric and telocentric markers

PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, G6PD, B, GLO-1, 1-2, Phenotype Frequency Product: 0.0115