SCLC-24H Cells

SCLC-24H is a small cell lung carcinoma (SCLC) cell line that exhibits characteristics typical of neuroendocrine tumors, which are aggressive and associated with poor prognosis. As with other SCLC models, SCLC-24H expresses a range of neuroendocrine markers such as neuron-specific enolase (NSE), L-DOPA decarboxylase (DDC), and creatine kinase-BB (CK-BB), which are used to differentiate SCLC from non-SCLC tumors. SCLC-24H also expresses carcinoembryonic antigen (CEA), a classical tumor marker, which is detectable in this cell line at levels higher than normal lung tissues.
In vitro studies on SCLC-24H have shown that this cell line is responsive to kinase inhibitors like staurosporine (SSP) and its analogs. These compounds can induce polyploidy and morphologic changes, such as process formation, characteristic of neuron-like differentiation in SCLC-24H. Staurosporine and related inhibitors like stauprimide and UCN-01 have been shown to influence the cell cycle of SCLC-24H by promoting cell flattening and sometimes process formation. However, SSPAs do not consistently induce extensive process outgrowth in SCLC-24H compared to other SCLC lines.
Additionally, like other SCLC cell lines, SCLC-24H demonstrates chromosomal abnormalities typical of small cell lung carcinoma, including deletions of the short arm of chromosome 3 (specifically at 3p14-23). These genetic alterations are often associated with the malignant behavior of SCLC cells. SCLC-24H, like other neuroendocrine tumors, can be a valuable model for studying therapeutic interventions targeting neuroendocrine markers and pathways such as PI3K/Akt or MAPK/ERK, which remain active in these cells.

Adherent

46 years

Caucasian

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Pleural effusion