Lama-84 Cells

LAMA-84 is a human cell line derived from the peripheral blood of a patient with chronic myeloid leukemia (CML) in blast crisis. This cell line is characterized by the presence of the Philadelphia chromosome, which results in the BCR-ABL fusion gene, a hallmark of CML. The BCR-ABL oncogene is known for its role in increasing tyrosine kinase activity, which promotes various signaling pathways leading to uncontrolled cell proliferation and resistance to apoptosis. LAMA-84 cells are thus an invaluable model for studying the molecular mechanisms of CML progression and for evaluating the efficacy of tyrosine kinase inhibitors (TKIs) in a pre-clinical setting.

In research, LAMA-84 has been extensively used to understand the biology of CML, especially in the context of drug resistance and disease evolution. Studies involving this cell line have helped in elucidating the cellular responses to different generations of TKIs, such as imatinib, dasatinib, and nilotinib. Moreover, LAMA-84 has contributed to the investigation of new therapeutic strategies aimed at overcoming TKI resistance, including the testing of combination therapies that target other signaling pathways synergistically affected by the BCR-ABL fusion protein.

Suspension, some adherent cells

29 years

Caucasian

1

EBNA, EA, and VCA were not detected

Supplement the medium with 10% FBS

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

1 to 2 x 10^4 cells/cm^2

BCR-ABL1 pos