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MML-1 Cells Europäischer Partner

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ArtNr CLS-300288
Hersteller CLS Cell Lines Service
Menge 1 cryovial
Kategorie
Typ Cell line
Zertifikat For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against Human (Homo sapiens)
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias MML1
Lieferbar
Manufacturer - Category
Skin cancer cell lines
Description
The MML-1 cell line is a melanoma cell line derived from malignant melanoma. This cell line is used primarily to study melanoma biology, particularly the role of extracellular vesicles (EVs) in cell-to-cell communication and tumor progression. MML-1 cells release various subtypes of EVs, including exosomes, microvesicles, and apoptotic bodies, each carrying distinct RNA cargos, such as microRNAs (miRNAs) and other non-coding RNAs.

Studies using MML-1 cells have demonstrated that exosomes released from these cells contain specific miRNAs like miR-214-3p, miR-199a-3p, and miR-155-5p, which are closely associated with melanoma progression and metastasis. These miRNAs are enriched in exosomes compared to other EV types and have been linked to important melanoma-related pathways, such as the regulation of the MAPK signaling pathway and tumor microenvironment interactions. Interestingly, comparisons of miRNA profiles from MML-1-derived exosomes with clinical samples of melanoma show a significant overlap, indicating the clinical relevance of this cell model in understanding melanoma progression.

In addition to miRNAs, MML-1 cells also release other non-coding RNAs such as small nucleolar RNAs (snoRNAs) and mitochondrial-associated transfer RNAs (mt-RNAs), which are differentially distributed among the EV subtypes. These findings highlight the MML-1 cell line's utility in studying the molecular mechanisms of melanoma, particularly how tumor cells communicate via EVs and influence their microenvironment.
Tissue
Skin
Growth properties
Adherent
Disease
Melanoma
Age
Unspecified
Gender
Unspecified
Morphology
Epithelial-like
Biosafety Level
1
Culture Medium
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium Supplements
Supplement the medium with 10% FBS
Subculturing
Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal
2 to 3 times per week
Freezing Recovery
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium
Handling of Cryopreserved Cultures

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions
When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer
Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty
We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Tumorigenic
Yes, in nude mice
Split Ratio
A ratio of 1:2 to 1:5 is recommended
Seeding Density
1 x 10^4 cells/cm^2
Reverse Transcriptase
negative
Mutational Profile
V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot).
Protein Expression
p53 positive

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 cryovial
Lieferbar: In stock
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