U2OS-CRISPR-NUP96-mMaple Cells

The U-2 OS-CRISPR-NUP96-mMaple is a genetically engineered osteosarcoma cell line derived from the human U-2 OS cell line, known for its robust growth characteristics and utility in various biological studies. This particular clone has been modified using CRISPR/Cas9 gene editing technology to incorporate mMaple, a photoconvertible fluorescent protein, into the NUP96 gene. The mMaple protein allows for advanced imaging techniques such as live-cell imaging and super-resolution microscopy, providing dynamic insights into the nuclear pore complex (NPC) behavior and cellular import-export mechanisms through the nuclear envelope.

The NUP96 gene, which encodes a crucial component of the NPC, is vital for nucleocytoplasmic transport. Alteration of NUP96 can affect not only transport mechanisms but also overall nuclear architecture and function. This cell line thus serves as an excellent model for studying NPC-related pathologies and the role of nuclear transport in cellular metabolism and signaling. The integration of mMaple into NUP96 permits real-time tracking and visualization of NUP96 dynamics in vivo, making it an indispensable tool for researchers focused on cell nucleus studies and those exploring the implications of NPC dysfunctions in diseases such as cancer and viral infections.

As a specialized tool, U-2 OS-CRISPR-NUP96-mMaple clone no.16 supports high-resolution imaging and provides substantial data regarding the spatial and temporal distribution of NPC components. It is particularly valuable for experiments requiring detailed analysis of gene expression, protein localization, and nuclear transport under physiological and pathological conditions, facilitating a deeper understanding of cellular processes at the molecular level.

Adherent

15 years

Caucasian

McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:3 to 1:6 is recommended

NUP96-mMaple (endogenous nuclear pore complex protein 96, mMaple tagged)