iPSC-hDPSC

Disease Modeling, Drug Discovery and Toxicity Testing, Regenerative Medicine, Genetic Research, Developmental Biology Studies

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately -78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

The iPSC-hDPSC cell line represents a cutting-edge model derived from human dental pulp stem cells (hDPSCs). These cells originate from dental pulp tissue, where they undergo enzymatic digestion to isolate the adherent fraction. Upon reaching 70-80% confluence, the cells are subcultured and cryopreserved at Passage 1. This foundational step is crucial in maintaining the cellular integrity and ensuring the viability of the stem cells for future applications.

The reprogramming of hDPSCs into induced pluripotent stem cells (iPSCs) is a significant advancement, achieved through an episomal reprogramming technique. This method employs vectors that incorporate the Yamanaka factors-Oct-4, Sox-2, and Klf-4-alongside p53 Anti-sense, EBNA-1, and a Red Fluorescent Protein marker. The episomal approach is particularly advantageous as it avoids the integration of reprogramming factors into the genome, thus preserving the genetic stability of the iPSCs. The resultant iPSC-hDPSC line exhibits pluripotent characteristics, making it a valuable tool for research in regenerative medicine, disease modeling, and drug discovery.

CLS Cell Lines Service

E8

Adherent

Normal donor

iPSC, colonies with defined edges

mTESR

Daily

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

iPSC-hDPSC (Cytion catalog number 300622)

Fast

CLS-860015, CLS-830100, CLS-831100