Human Mesenchymal Stem Cells - Bone Marrow (HMSC-BM)

Drug testing, regenerative medicine, disease research

Human Mesenchymal Stem Cells derived from Bone Marrow (HMSC-BM) represent a robust and versatile tool for in vitro research. These multipotent mesenchymal stromal cells (MSCs) possess the unique ability to self-renew and differentiate into a broad spectrum of cell types, including adipocytes, osteoblasts, and chondrocytes. The potential of HMSC-BM to differentiate into these three key lineages has been well-documented, making them invaluable for studies focused on regenerative medicine, tissue engineering, and cellular differentiation pathways. These MSCs are cultivated under stringent conditions, ensuring their multipotency and high viability post-thaw.

One of the distinguishing features of HMSC-BM compared to MSCs derived from other sources, such as adipose tissue or umbilical cord, is their superior capacity for osteogenic differentiation. This makes them particularly useful in bone biology and orthopedic research, where understanding the molecular mechanisms governing bone formation and repair is crucial. Additionally, HMSC-BM exhibit a robust immunomodulatory profile, which makes them an excellent model for studying immune interactions and inflammatory responses. These unique characteristics also position HMSC-BM as a preferred choice for preclinical studies exploring bone marrow microenvironment, hematopoiesis, and the pathophysiology of bone marrow-related diseases.

Each cryovial of HMSC-BM contains a minimum of 1 x 106 cells, with viability rates ranging between 92% to 95%, as determined by the Trypan Blue dye exclusion test. These cells are derived from bone marrow collected from healthy adult donors, all of whom have provided informed consent. To ensure the highest standards, each batch undergoes rigorous quality control testing to assess cell identification, purity, potency, and viability. This thorough testing guarantees that the MSCs meet strict criteria, making them suitable for a wide range of research applications, including cell biology studies, drug discovery, and the investigation of cellular responses to different stimuli. These cells are not intended for therapeutic or in vivo applications, and their use is confined to research purposes in a controlled laboratory environment.

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Caucasian

1

Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

1 to 3 x 10^3 cells/cm^2

Trypsin-EDTA