MH-3924A Cells

The MH3924A cell line is a well-characterized model derived from Morris rat hepatoma 3924A, which is frequently used in research to study hepatocellular carcinoma (HCC). These cells have been extensively employed to investigate the mechanisms underlying HCC growth, metastasis, and therapeutic responses. In particular, MH3924A cells are noted for their robust proliferative capacity and their ability to invade surrounding tissues, making them a suitable in vitro and in vivo model for exploring cancer progression and potential treatments.
Studies have demonstrated that the proliferation and invasiveness of MH3924A cells can be significantly influenced by various factors. For instance, treatment with the immunosuppressive drug tacrolimus (FK506) has been shown to promote the proliferation of these cells, enhance their invasive potential, and increase the expression of key molecules involved in metastasis, such as CXCR4 and its ligand SDF-1α. FK506's effect on these cells underscores its potential to exacerbate cancer progression, particularly in the context of post-transplantation immunosuppression, where its use is common to prevent organ rejection but may inadvertently promote tumor growth.
Additionally, MH3924A cells have been genetically modified to express the human sodium/iodide symporter (hNIS), which significantly enhances their iodide uptake capability. This modification has facilitated the use of these cells in radioiodine therapy studies, providing insights into the potential application of gene therapy for targeting HCC. However, despite the increased uptake, the rapid efflux of iodide from the cells suggests that further modifications or combined treatments are necessary to retain the radioactivity within the tumor cells for effective therapy. The MH3924A cell line thus remains a pivotal model in both basic and applied cancer research, particularly in the study of HCC's molecular underpinnings and therapeutic strategies.

Adherent

16 months

ACI

1

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

Start culture using the complete contents of the cryovial in 2xT25 cell culture flasks. The cells will recover within 24 to 48 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in ACI-rat

2 x 10^4 cells/cm^2