AR42J Cells

AR42J cells are a rat pancreatic tumor cell line derived from azaserine-induced tumors in rats. They are widely used as a model for studying pancreatic exocrine cell functions, pancreatitis, and pancreatic cancer research. AR42J cells exhibit acinar-like characteristics, making them particularly valuable for investigating the physiology and pathology of pancreatic acinar cells.
One of the defining features of AR42J cells is their ability to differentiate into cell types exhibiting more pronounced pancreatic exocrine functions when treated with various agents, such as dexamethasone or activators of protein kinase C. Upon differentiation, these cells produce and secrete digestive enzymes, including amylase, lipase, and chymotrypsin, mimicking the enzyme secretion profile of normal pancreatic acinar cells.
AR42J cells are also used to explore the mechanisms of acute pancreatitis. They respond to stimuli like cerulein, a cholecystokinin analog, which can induce a condition in the cells that resembles acute pancreatitis, characterized by enzyme overproduction, oxidative stress, and inflammatory responses. This makes AR42J cells a useful tool for testing potential therapeutic interventions for pancreatitis.
Furthermore, the AR42J cell line is utilized in research focused on pancreatic cancer, particularly for studies on tumorigenesis and the malignant transformation of acinar cells. They are instrumental in examining the effects of oncogenes, tumor suppressor genes, and growth factors on the development and progression of pancreatic cancer.
Overall, AR42J cells provide a versatile and dynamic model system for advancing our understanding of pancreatic diseases and for the development of new therapeutic strategies targeting these conditions.

Cells grow slowly, in clusters and appear as hollow spheroid colonies. They can pile up and attach loosely.

Epithelial-like

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

It is recommended to cover the tissue culture flasks with gelatine prior to cell cultivation. Gelatine is added to the flask, incubated for 30min at 37 degree Celsius and washed once with PBS. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

1 x 10^4 cells/cm^2

Insulin, glucocorticoid