NRK-Pom121-EGFP3 Cells

The NRK-Pom121-EGFP3 cell line is derived from normal rat kidney (NRK) cells and is genetically engineered to express the Pom121-EGFP3 fusion protein. Pom121 is a transmembrane nucleoporin that is an integral component of the nuclear pore complex (NPC), playing a crucial role in nuclear envelope assembly and NPC function. The inclusion of the enhanced green fluorescent protein (EGFP3) tag facilitates the visualization and study of Pom121 dynamics, localization, and interactions within live cells through fluorescence microscopy. This makes the NRK-Pom121-EGFP3 cell line a valuable tool for investigating nuclear transport mechanisms and NPC architecture.

NRK cells, the parental line of NRK-Pom121-EGFP3, are commonly used in various research applications due to their stable growth characteristics and epithelial morphology. The modification to express Pom121-EGFP3 provides researchers with a robust model to examine the molecular mechanisms underlying nucleocytoplasmic transport, the structural organization of the NPC, and its regulation during cell division and differentiation. Additionally, this cell line can be used to study the effects of various genetic and pharmacological perturbations on NPC function, offering insights into diseases associated with nuclear transport defects, such as cancer and neurodegenerative disorders.

Overall, the NRK-Pom121-EGFP3 cell line represents a sophisticated tool in cell biology and molecular research, providing high-resolution insights into the dynamic processes governing nucleocytoplasmic interactions. Its ability to allow real-time observation of NPC components in a live cellular context makes it invaluable for advancing our understanding of cellular transport mechanisms and their implications in health and disease.

Monolayer, adherent

Fibroblast-like cells with fusiform shape

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Discard the old medium and wash the cells with PBS. Add a freshly prepared 0.025% trypsin/0.02% EDTA solution heated to 37 degrees Celsius and wait until the cells detach, which usually takes about 5 minutes. Neutralize the trypsin by adding fresh medium, then transfer the cell mixture to a tube and centrifuge. After centrifugation, remove the supernatant, resuspend the cell pellet in fresh culture medium, and transfer the suspension to new flasks. Incorporate G418 into the culture medium to achieve a final concentration of 0.5 mg/ml

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:3 to 1:4 is recommended

Epidermal growth factor (EGF), multiplication stimulating activity (MSA), POM121, Transmembrane, Nucleoporin, CMV Promotor, Neomycin, Phosphotransferase

Epidermal growth factor (EGF), multiplication stimulating activity (MSA)