NRK-EGFP-H2B Cells

The NRK-EGFP-H2B cell line is a genetically modified variant of normal rat kidney (NRK) cells that stably express the enhanced green fluorescent protein (EGFP) fused to histone H2B. This modification allows for real-time visualization of chromatin and nuclear dynamics, making this cell line an invaluable tool for studying cell cycle progression, mitosis, and chromatin organization. The stable expression of EGFP-H2B provides a bright and consistent fluorescent signal, facilitating high-resolution live-cell imaging and enabling researchers to monitor nuclear events with great precision.

NRK cells, originating from the kidney tissue of an adult rat, are widely used in cellular biology due to their robust growth characteristics and well-documented physiological behaviors. The introduction of the EGFP-H2B fusion protein into these cells does not significantly alter their growth or morphology, allowing for reliable and reproducible experimental conditions. This cell line is particularly useful in studies of kidney cell biology, cellular responses to stress, and mechanisms of carcinogenesis, given the kidney's role in filtering blood and excreting waste. Additionally, the fluorescence capabilities of NRK-EGFP-H2B cells can be harnessed in drug screening applications to observe drug effects on cell proliferation and nuclear morphology in real-time.

Monolayer, adherent

Fibroblast-like cells with fusiform shape

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Discard the old medium and wash the cells with PBS. Add a freshly prepared 0.025% trypsin/0.02% EDTA solution heated to 37 degrees Celsius and wait until the cells detach, which usually takes about 5 minutes. Neutralize the trypsin by adding fresh medium, then transfer the cell mixture to a tube and centrifuge. After centrifugation, remove the supernatant, resuspend the cell pellet in fresh culture medium, and transfer the suspension to new flasks. Incorporate G418 into the culture medium to achieve a final concentration of 0.5 mg/ml

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:3 to 1:4 is recommended

Epidermal growth factor (EGF), multiplication stimulating activity (MSA), CMV Promotor Histone H2B, Neomycin, Phosphotransferase

Epidermal growth factor (EGF), multiplication stimulating activity (MSA)