LMH Cells

The cell line is useful for transfection studies.

LMH cells, derived from a Leghorn male hepatoma, are a versatile cell line widely used in biological research. Tomoyuki Kitagawa established them in 1981 at the Cancer Institute in Tokyo, Japan. These cells have an epithelial phenotype and are particularly useful for studying host-pathogen interactions in the gastrointestinal tract of poultry.
LMH cells are adherent and exhibit a dendritic-like morphology. They express glucose-6-phosphatase and weak canalicular ATPase activity. With a triploid karyotype and six marker chromosomes, these cells display distinct genetic characteristics.Notably, LMH cells have been shown to efficiently support duck hepatitis B virus (DHBV) DNA synthesis when transfected with viral constructs. This makes them an invaluable tool for virology research, particularly in the context of poultry-related viral infections.The derivation of LMH cells involved inducing tumorous nodules in the liver of Leghorn chickens through long-term treatment with diethylnitrosamine. These cells have also been chemically transformed, allowing for their immortalization and continuous propagation in culture.
In terms of tumorigenicity, LMH cells have the ability to form tumors in athymic nude mice. This characteristic makes them an important model for studying hepatocellular carcinoma. LMH cells express the estrogen receptor and can be induced to express the liver-specific apolipoprotein II (apoII) gene. This indicates their involvement in estrogen signaling pathways and lipid metabolism. To culture LMH cells, it is necessary to precoat tissue culture vessels with 0.1% gelatin. This ensures proper cell adhesion and growth.

Adherent. It may take a couple of days until cells grow in fully adherent colonies.

16 months

Leghorn

1

Supplement the medium with 10% FBS

Every 2 days

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

LMH cells form tumors in athymic mice.

1 to 3 x 10^6 cells/cm^2

triploid, modal number = 116, six marker chromosomes