CHO-K1 Cells

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Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

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ArtNr	CLS-603480
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Zertifikat	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Hamster
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CHO K1,CHOK1,CHO cell clone K1,GM15452
Lieferbar
Manufacturer - Applications	This cell line is an optimal choice for toxicology, industrial biotechnology and bioproduction.
Manufacturer - Category	Hamster cell lines
Description	CHO-K1 cells are a subline derived from the CHO cell line, which was originally established in the early 1950s from a Chinese hamster ovary. CHO-K1 cells are widely utilized in the production of therapeutic monoclonal antibodies and other biopharmaceuticals. Their extensive use in biopharmaceutical protein production and vaccines is attributed to their eukaryotic nature, which allows for proper folding, assembly, and post-translational modifications such as glycosylation, which influences the stability, efficacy, and safety of the produced proteins. In the realm of recombinant protein production, the CHO-K1 cell line is used to express a wide array of proteins, including monoclonal antibodies, growth factors, cytokines, and enzymes. These proteins have applications in therapeutic treatments, diagnostic assays, and vaccine formulations. CHO-K1 cells exhibit a robust growth rate and are adaptable to various culture conditions, including suspension and adherent cultures, making them highly valuable for large-scale bioproduction processes. They possess a high level of genetic stability and are used for stable cell line development as they are capable of amplifying and expressing exogenous genes efficiently, which is critical for producing high yields of recombinant proteins. CHO-K1 chinese hamster cells can be easily transfected with a variety of vectors for gene expression, facillitating gene editing or knockdown. This flexibility allows researchers to introduce specific genes, silence genes, or even perform targeted gene editing using technologies like CRISPR-Cas9 in CHO-K1 host cells. In conclusion, the chinese hamster CHO-K1 cells and CHO cells are pivotal in biotechnological research and biopharmaceutical production, offering a versatile platform for the study of gene function and the large-scale production of recombinant proteins.
Tissue	Ovary
Growth properties	Monolayer, adherent
Age	Adult
Gender	Female
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Split Ratio	A ratio of 1:4 to 1:8 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will yield in a confluent layer in about 6 days
Virus Susceptibility	vesicular stomatitis (Indiana), Getah virus Virus Resist: poliovirus 2, modoc virus, Button Willow virus
Doubling Time	22 hours
Reverse Transcriptase	negative
Karyotype	Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 1 cryovial

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