PtK2 Cells

PtK2 cells are an epithelial cell line derived from the kidney of a male long-nosed potoroo, Potorous tridactylis, a marsupial species. These cells are well-known for their large size and the small number of chromosomes (2n = 12), making them particularly useful in cytogenetic studies. Due to their easily visualized chromosomes, PtK2 cells serve as an excellent model for studying mitosis, chromosome movement, and the structural aspects of cell division. Additionally, they maintain a flat morphology throughout the cell cycle, including during mitosis, which aids in the observation of cellular processes under microscopy. PtK2 cells exhibit specific virus susceptibility patterns, being resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2, while being susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis viruses. Furthermore, these cells possess intermediate filaments composed of keratin, which contribute to their structural integrity. In biomedical research, PtK2 cells are often utilized in the study of cell division, virus-host interactions, and cytoskeletal organization.

Monolayer, adherent

Male

1

Supplement the medium with 10% FBS

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

A ratio of 1:2 to 1:3 is recommended

Coxsackievirus A9, herpes simplex, vaccinia, vesicular stomatitis (Ogden)

Keratin