ME-180 Cells

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Profit Organisation/Nutzung: CLS_CLEAR.pdf
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ArtNr	CLS-300196
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Zertifikat	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Me-180,ME 180,ME180
Lieferbar
Manufacturer - Category	Reproductive system cancer cell lines
Description	The ME-180 cell line is an epithelial cell line established from a highly invasive squamous cell carcinoma, originally isolated from the omental metastasis of a cervical carcinoma in a 66-year-old White female patient. The carcinoma was characterized by irregular cell clusters with no significant keratinization and minimal necrosis. This cell line is particularly significant for cancer research, especially in studies involving cervical cancer and other forms of squamous cell carcinoma, due to its origin and aggressive nature. ME-180 cells are tumorigenic and have been shown to form well-differentiated epidermoid carcinomas when implanted in nude mice. ME-180 cells have several unique properties, including a heteroploid karyotype with a subtriploid mode, indicating an unstable chromosomal arrangement. The cells exhibit typical epithelial morphology with numerous desmosomes and tonofibrils, and they do not exhibit contact inhibition, often leading to layered growth in culture. The cell line's growth is inhibited by tumor necrosis factor alpha (TNF alpha), making it useful for studies investigating the effects of inflammatory cytokines on tumor cells. Additionally, ME-180 cells contain human papillomavirus (HPV) DNA, with a higher homology to HPV-68 compared to HPV-18, which could be relevant for studies on HPV-related carcinogenesis. ME-180 cells are also valuable in infectious disease research due to their sensitivity to various viruses. The cell line has been used to study the interaction with several viruses, including influenza and myxoviruses. ME-180 cells have shown the ability to form persistent infections with some myxoviruses, making them a useful model for studying viral latency and the long-term effects of viral infection on cancer cells. The combination of its cancerous origin, viral susceptibility, and specific growth characteristics make ME-180 a versatile tool in both oncology and virology research.
Tissue	Uterus, Cervix
Growth properties	Adherent
Cell type	Epithelial
Disease	Epidermoid Carcinoma
Age	66 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Viruses	HPV positive
Culture Medium	McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, X
Seeding Density	1 x 10^4 cells/cm^2
Metastatic Site	Omentum
Passaging Solution	Accutase

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Menge: 1 cryovial

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