Human Mesenchymal Stem Cells - Umbilical Cord - Artery

Drug testing, regenerative medicine, disease research

Human Mesenchymal Stem Cells (hMSCs) derived from the umbilical cord artery are a distinct and promising subtype of mesenchymal stem cells, offering several unique advantages over other MSC sources. Unlike MSCs derived from bone marrow or adipose tissue, umbilical cord artery MSCs are harvested from a more primitive and less invasive source, providing a younger and potentially more potent cell population. This origin confers a higher proliferative capacity and longer telomeres, which may enhance their self-renewal abilities and reduce the risk of senescence during extended culture. Furthermore, MSCs from the umbilical cord artery typically express a unique set of surface markers and have a lower immunogenic profile, making them particularly suitable for allogeneic applications and reducing the risk of immune rejection.

In vitro, umbilical cord artery-derived MSCs demonstrate robust multipotency, with the ability to differentiate into adipocytes, osteoblasts, and chondrocytes when exposed to specific differentiation media. This versatility is comparable to that of MSCs derived from other tissues, but with the added benefit of their primitive nature, which may enhance their therapeutic potential. Each batch of these MSCs undergoes stringent quality control, including assessments for viability, purity, and potency, ensuring that the cells meet high standards for research applications. The cells are cryopreserved at early passages using a specialized cryomedium, maintaining their high viability (92% to 95%) upon thawing, which is crucial for their effective use in downstream applications.

Overall, hMSCs from the umbilical cord artery offer a combination of easy accessibility, high proliferative capacity, and low immunogenicity, making them a valuable tool for a wide range of research studies, particularly those focusing on regenerative medicine and immune modulation. These cells, harvested with full donor consent, represent a high-quality and ethically sourced option for researchers looking to explore the full potential of mesenchymal stem cells in vitro.

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Caucasian

1

Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

1 to 3 x 10^3 cells/cm^2

Trypsin-EDTA