CT26.WT Cells

CT26.WT is a clonally derived cell line from the parent CT26 cell line, which itself was established from a colon carcinoma induced in a BALB/c mouse using the carcinogen N-nitroso-N-methylurethane (NNMU). This cloning process was performed to obtain a cell line with consistent characteristics and reproducible results in experimental setups. As a result, CT26.WT retains the undifferentiated carcinoma phenotype of its progenitor, making it a robust model for studying various aspects of colorectal cancer, including tumor genesis, progression, and the tumor microenvironment.
This cell line is extensively used in oncological research, particularly in the study of immune responses to tumors. Its compatibility with BALB/c mice, which are genetically identical to the source of the CT26.WT cells, allows researchers to study the complex interactions between cancer cells and the immune system in a controlled yet biologically relevant setting. The use of CT26.WT in syngeneic murine models helps in the investigation of immunotherapeutic strategies, such as the efficacy of novel immunomodulatory agents and the role of immune checkpoints in cancer progression. This facilitates the development of more effective cancer treatments that can later be adapted for human clinical trials.

Adherent

BALB/c

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

1:2 to 1:4