L6565 Cells

L6565 cells were derived from pancreatic suspensions of splenocytes from L6565 leukemia mice. The chromosomal count ranged from 38 to 144. Electron microscopic observations revealed that the clonal L6565 cells had well-defined nuclei and an abundance of organelles and Class A and Class C viral particles in the cytoplasm. The oncogenes c-myc and c-fos were overexpressed in these cells. The L6565 cell clone is an RNA virus-containing lymphoblastic leukemia stem cell line. It has passed the mycoplasma detection test in this library.


The significance of the L6565 cell line lies in its provision of standardized experimental cell resources and associated technical support for research in the fields of life sciences and biotechnology. These cells can be crucial for understanding the molecular mechanisms of leukemia, particularly the role of viral particles and oncogene expression in leukemogenesis. Additionally, they serve as a valuable tool for drug testing and development, allowing researchers to explore potential therapeutic strategies for leukemia and other related disorders.

Suspension

1

Supplement the medium with 10% FBS, 0.005 mg/mL insulin, 0.01 mg/mL human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, bovine pituitary extract (0.15 mg protein per ml)

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.