Y-27632 Dihydrochloride

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCepsilon than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (ca.100 µ M). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC50 = 700 nM for phenylephrine-induced contractions in aortic strips). Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency.

146986-50-7

C¹⁴H²¹N³O&bull, 2HCL&bull, H²O

White solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS.