Y-27632

146986-50-7

C14H21N3O

DMSO : 50 mg/mL (202.15 mM; Need ultrasonic)

TGF-beta/Smad; Stem Cell/Wnt; Cell Cycle/DNA Damage

Y-27632 is an ATP-competitive inhibitor of ROCK-I and ROCK-II, with K_i of 220 nM and 300 nM for ROCK-I and ROCK-II, respectively, which primes human induced pluripotent stem cells (hIPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation. IC50 & Target: Ki: 220/300 nM (ROCK-I/II)^[1] In Vitro: Y-27632 inhibits the ROCK family of kinases 100 times more potently than other kinases including protein kinase C, cAMP-dependent kinase and myosin light chain kinase. Y-27632 prolongs the lag time and delays the appearance of BrdU-labeled cells in a concentration-dependent manner, delays of about 1 and 4 h are noticed in the Swiss 3T3 cells treated with 10 and 100 uM Y-27632, respectively^[1]. Y-27632 promotes neuronal differentiation of adipose tissue-derived stem cells (ADSCs). Compared to 1.0 and 2.5 uM Y-27632 induced groups, percentages of neuroal-like cells achieved a peak in the 5.0 uM Y-27632 induced group^[2]. In Vivo: Y-27632 (5 and 10 mg/kg) significantly prolongs the onset time of myoclonic jerks when compare with saline group. Y-27632 (5 and 10 mg/kg) significantly prolongs the onset time of clonic convulsions when compare with saline group^[3]. Treatment with Dimethylnitrosamine (DMN) causes a significant decrease in rat body and liver weight (DMN-S group) compared with control animals (S-S group). Oral Y27632 (30 mg/kg) essentially prevents this DMN-induced rat body and liver weight loss (DMN-Y group)^[4].