JC-1

3520-43-2

C25H27Cl4IN4

H2O : < 0.1 mg/mL (insoluble); DMSO : >= 15 mg/mL (23.00 mM)

Others

JC-1 is a fluorescent lipophilic carbocyanine dye, which can be used to measure mitochondrial membrane potential. In Vitro: JC-1 (2.5?uM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488?nm laser wavelength common in flow cytometers^[1]. Fluorescent labeling of mitochondria with either JC-1 (1 ug/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes^[2]. JC-1 has been shown to interact with alpha-synuclein at the acidic C-terminal region with a K_d of 2.6 uM. JC-1 itself does not accelerate the protein aggregation of alpha-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx^[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence^[4].